Parasitological investigations of clinical specimens

Specimen	Procedure	Amount of specimen	Conditions of transportation and storage	Testing results obtained in (days)
Fresh stool	Determination of ova and parasite (O&P) by Kato-Katz technique	≥ 5–10g	At room temperature –1 h, at 2–8°C – 24 h	1–2
Fresh stool	Determination of intestinal protozoa by direct smear. Intended for diarrhea patients.	≥ 5–10g	Stool must be delivered within 15–30 min. to check trophozotes motility	1
Fresh or formalin (10%) preserved stool	Determination ofova and parasite (O&P) by formalin-ethyl acetate sedimentation technique	≥ 5–10g	Fresh: at room temperature – 1 h, at 2–8 C – 24 h. Formalin preserved at room temperature – up to 7 days.	1–2
Fresh or PVA preserved stool	Determination of intestinal protozoan trophozoites and cysts by technique of permanent smear stained with Trichrome	≥ 5–10g	Fresh: at room temperature – 1 h. PVA preserved at room temperature – up to 7 days.	1–3
Fresh or formalin preserved stool	Determination of Cryptosporidium, Cyclospora and Isospora (intestinal protozoa) in permanent smear stained by modified Kinyoun's acid-fast technique	≥ 5–10g	Fresh: at room temperature – 1 h. Formalin preserved at room temperature – up to 7 days.	1–2
Fresh stool		≥ 5–10g	At room temperature –1 h, at 2–8°C – 24 h	1–3
Fresh stool	Determination of Entamoeba histolytica antigen by ELISA or immunochromatographic assay technique	≥ 5–10g	At room temperature – 1 h, at 2–8°C – 24 h	1–3
Fresh stool	Determination of Cryptosporidium antigen by ELISA or immunochromatographicassay technique	≥ 5–10g	At room temperature – 1 h, at 2–8°C – 24 h	1–3
Perianal swab	Determination of pinworm (Enterobius vermicularis) or cestoda eggs on cellulose tape	1 slide	At room temperature – 24 h, at 2–8°C – 3 days	1–2
Cyst aspirates	Determination of Echinoccocus scoleces and hooklets	≥ 5 ml	At room temperature – 1 h, at 2–8°C – 24 h	1–2
Thin and thick blood smears	Determination of Malaria and other blood parasites	1. 2 thin and 2 thick blood smears; 2. 5 ml venous blood in tube with EDTA	At room temperature – as soon as possible	1
In case of tropical parasitic disease and other special requests – contact the reception. Note: one teaspoon contains from 5 to 10 g of stool.

Nacionalinė visuomenės sveikatos priežiūros laboratorija
Žolyno g. 36 LT-10210 Vilnius.
Biudžetinė įstaiga, kodas 195551983, duomenys kaupiami ir saugomi Juridinių asmenų registre

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Klientų aptarnavimas Vilniuje

Tel. nr. +370 672 94 184,
+370 5 234 4003
El. p. nvspl@nvspl.lt

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