Parasitological investigations of environmental specimens

SpecimenProcedureAmount of specimenConditions of transportation and storageTesting results obtained in (days)
Environmental scrapingsMicroscopic examination by Grechem technique2*7 cm adhesive cellulose tape

At room temperature - 8 h,
at 2–8°C – 24 h.

Shipment of specimens – in microscope slides box

1–2
SoilDetermination of helminth eggs and larvae by flotation technique150–200 g

At room temperature – 8 h,
at 2–8°C – 24 h.

Shipment of specimens – in plastic containers

1–3
SludgeDetermination of helminth eggs and larvae by flotation technique150–250 ml

At room temperature – 8 h,
at 2–8°C – 24 h.

Shipment of specimens – in plastic or glass containers

1–3
Fruit and vegetableDetermination of helminth eggs and larvae by flotation and sedimentation technique0,5–1 kg

At room temperature – 8 h,
at 2–8°C – 24 h.

Shipment of specimens – in plastic containers

1–3
WaterDetermination of helminth eggs and larvae by sedimentation technique10–50 l of pool water;
3–5 l of well, river, lake or pond water

At room temperature, in the dark – 8 h,
at 2–8°C – 24 h.

Shipment of specimens – in plastic containers

2–4
Water (potable, pool)Determination of Cryptosporidium and Giardia by sedimentation technique10–50 l of pool
or potable water

At room temperature, in the dark – 8 h,
at 2–8°C – 24 h.

Shipment of specimens – in plastic containers

2–4

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